The insect-killing bacterium Photorhabdus luminescens has the lowest mutation rate among bacteria

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Measuring virulence factor expression by the pathogenic bacterium Photorhabdus luminescens in culture and during insect infection.

During insect infection Photorhabdus luminescens emits light and expresses virulence factors, including insecticidal toxin complexes (Tcs) and an RTX-like metalloprotease (Prt). Using quantitative PCR and protein assays, we describe the expression patterns of these factors both in culture and during insect infection and compare them to the associated bacterial growth curves. In culture, light a...

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Isolation and Identification of Photorhabdus Luminescens an Insect Pathogenic Bacteria Associated with Entomopathogenic Nematode

Photorhabdus luminescens is a gram negative symbiotic bacterium belonging to the family Enterobacteriaceae, and is a lethal pathogen of insects. It lives in the gut of an entomopathogenic nematode of the family Heterorhabditidae. When the nematode infects an insect, P. luminescensis released into the blood stream and rapidly kills the insect host by producing toxins. So the present study was ca...

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Mathematical model of the Lux luminescence system in the terrestrial bacterium Photorhabdus luminescens.

A mathematical model of the Lux luminescence system, governed by the operon luxCDABE in the terrestrial bacterium Photorhabdus luminescens, was constructed using a set of coupled ordinary differential equations. This model will have value in the interpretation of Lux data when used as a reporter in time-course gene expression experiments. The system was tested on time series and stationary data...

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A metalloprotease secreted by the insect pathogen Photorhabdus luminescens induces melanization.

Photorhabdus luminescens is a gram-negative insect pathogen that enters the hemocoel of infected hosts and produces a number of secreted proteins that promote colonization and subsequent death of the insect. In initial studies to determine the exact role of individual secreted proteins in insect pathogenesis, concentrated culture supernatants from various P. luminescens strains were injected in...

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A zinc metalloprotease inhibitor, Inh, from the insect pathogen Photorhabdus luminescens.

The entomopathogen Photorhabdus luminescens secretes many proteins during the late stages of insect larvae infection and during in vitro laboratory culture. The authors have previously characterized and purified a 55 kDa zinc metalloprotease, PrtA, from culture supernatants of P. luminescens. PrtA is secreted via a classical type I secretory pathway and is encoded within the operon prtA-inh-prt...

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ژورنال

عنوان ژورنال: Marine Life Science & Technology

سال: 2020

ISSN: 2096-6490,2662-1746

DOI: 10.1007/s42995-020-00060-0